Scientist/Associate Scientist, Antibody Discovery – R&D
Elevian is a well-funded early stage biotechnology company developing transformative therapeutics to address diseases of aging. Elevian’s founders, Drs. Richard T. Lee, Amy Wagers and Lee Rubin, in the Harvard Department of Stem Cell Biology and Regenerative Biology (HSCRB) have identified specific circulation blood factors that promote regeneration in tissues that have been damaged by aging and degeneration. Mouse model studies involving supplementation of GDF11, a TGF-b ligand, have demonstrated improvements in age related cardiac hypertrophy, youthful skeletal muscle repair, neurogenesis and cognitive function, and Type II Diabetes. This ground-breaking discovery was recognized by Science as a Global Top 10 Breakthrough of 2014. Elevian is developing therapeutics that regulate GDF11 and other circulating factors that stimulate and replenish our body’s natural regenerative capacity to address multiple degenerative diseases. We are looking for well qualified, enthusiastic, and motivated scientists who wish to join the Elevian team of serial entrepreneurs, biotech veterans, and world renowned scientists in regenerative medicine and aging biology and contribute to our mission to develop therapeutics that enable “healthy aging”. Elevian’s research is being performed at The Pagliuca Harvard Life Lab to enable close collaboration with the laboratories of its academic founders.
Position Description: Scientist/Associate Scientist Antibody Discovery – R&D
We are seeking dedicated, innovative, and motivated Scientists, Associate Scientists or Senior Research Associates with extensive hands on experience in antibody discovery & engineering including antibody expression and purification from stable cell lines. The successful candidate will be a key contributor to the evaluation and selection to the discovery and advancement of Elevian’s regenerative biotherapeutics (recombinant proteins and antibodies) that promote “healthy aging” by modulating the TGF-b pathway.
Responsibilities of the successful candidate will include:
Laboratory activities related to antibody discovery and optimization campaigns.
Screening of antibodies from hybridoma and phage display campaigns using biochemical and cell-based assays.
Antibody sequencing (V region), recombinant antibody expression, purification and detailed characterization.
Stable cell line generation for antibody/protein expression in mammalian suspension cell culture systems (HEK293 and CHO).
Develop and implement biochemical and cell-based screening assays for antibody therapeutic lead selection
Develop and implement assays to characterize antibody and antigen activity and function in vitro.
Characterization of potency and signaling activity of candidate therapeutics in vitro and support selection of candidates for in vitro and in vivo efficacy studies.
Collaborate with labs of Elevian’s three academic founders at HSCRB and with third party CROs
Contribute to the culture and team leadership at Elevian’s research labs at The Pagliuca Harvard Life Lab
PhD/MS (0-3 years’ experience) or BS (3-5 years’ experience) with a background in antibody discovery, immunology, or protein biochemistry in industry or academia.
Hands-on experience with antibody and/or protein binding biochemical/biophysical assays (BLI on Fortebio Octet and/or SPR on Biacore platforms).
Experience with transient transfection and stable mammalian (CHO and HEK) generation for protein and/or antibody production.
Experience in antibody and/or protein purification (affinity, ion-exchange, size-exclusion) column chromatography methods.
Experience with standard cell and molecular biology techniques and assays including Western Blots, ELISA, qPCR, RNAseq, and cDNA cloning.
Experience with flow cytometry and FACS analysis is a plus.
Experience with antibody sequence isolation, repertoire analysis, and V region classification highly preferred.
Experience with developability assessment (CIC, AC-SINS, BV ELISA) of antibody-therapeutics highly preferred.
Ability to work independently, design experiments, follow and adapt protocols, conduct, interpret, and optimize experimental studies.
Excellent verbal and written communication skills.
Clear scientific presentation skills during project and team meetings.
Excellent organizational and documentation skills: expected to document all aspects of experiments performed and be able to conduct independent research to collect and archive all necessary information from the literature.
Excellent team working skills and adaptability/flexibility.