Scientist/Associate Scientist/Sr. Research Associate, Cell Biology – R&D
Elevian is a well-funded early stage biotechnology company developing transformative therapeutics to address diseases of aging. Elevian’s founders, Drs. Richard T. Lee, Amy Wagers and Lee Rubin, in the Harvard Department of Stem Cell Biology and Regenerative Biology (HSCRB) have identified specific circulation blood factors that promote regeneration in tissues that have been damaged by aging and degeneration. Mouse model studies involving supplementation of GDF11, a TGF-b ligand, have demonstrated improvements in age related cardiac hypertrophy, youthful skeletal muscle repair, neurogenesis and cognitive function, and Type II Diabetes. This ground-breaking discovery was recognized by Science as a Global Top 10 Breakthrough of 2014. Elevian is developing therapeutics that regulate GDF11 and other circulating factors that stimulate and replenish our body’s natural regenerative capacity to address multiple degenerative diseases. We are looking for well qualified, enthusiastic, and motivated scientists who wish to join the Elevian team of serial entrepreneurs, biotech veterans, and world renowned scientists in regenerative medicine and aging biology and contribute to our mission to develop therapeutics that enable “healthy aging”. Elevian’s research is being performed at The Pagliuca Harvard Life Lab to enable close collaboration with the laboratories of its academic founders.
Position Description: Scientist/Associate Scientist/Sr. Research Associate, Cell Biology – R&D
We are seeking dedicated, innovative, and motivated Scientists, Associate Scientists or Senior Research Associates with extensive hands on experience in cell biology and/or antibody discovery, receptor pharmacology, cell signaling assays, and culture of multiple cell types including stable cell lines, stem cells, and primary cells (e.g. neuronal, muscle, and endothelial cells). The successful candidate will be a key contributor to the evaluation and selection to the discovery and advancement of Elevian’s regenerative biotherapeutics (recombinant proteins and antibodies) that promote “healthy aging” by modulating the TGF-b pathway.
Responsibilities of the successful candidate will include:
Stable cell line generation for protein and antibody expression in mammalian suspension cell culture systems (CHO and HEK).
Develop and implement assays to characterize protein activity and function in vitro.
Characterize the potency and signaling activity of candidate recombinant proteins and antibodies in vitro and support selection of candidates for in vitro and in vivo efficacy studies.
Generate stable cell lines (CRISPR knockout, overexpression) to be used in cell-based assays and to create disease-relevant cell models.
Develop and implement assays to evaluate tissue specific cell signaling of candidate recombinant proteins and antibodies both in primary cells (e.g. neuronal, muscle, endothelial cells etc.)
Development of cell based and/or biochemical approaches and technologies to establish the activity and mechanism of action of candidate therapeutics.
Collaborate with labs of Elevian’s three academic founders at HSCRB and with third party CROs.
Contribute to the culture and team leadership at Elevian’s research labs at The Pagliuca Harvard Life Lab.
Ph.D/MS (0-3 years’ experience) or BS with (2-4 years’ experience) at industry or academia.
Must have extensive experience with mammalian cell culture and aseptic techniques.
Experience with transient transfection and stable mammalian (CHO and HEK) cell line generation is required.
In-depth knowledge of cell-based activity-reporter assays and other cell signaling assays and formats (FP, FRET, etc.) is a must.
Experience with standard cell and molecular biology techniques and assays including ELISA, Western Blots, immunohistochemistry, qPCR, cloning in mammalian expression vectors, and RNAseq will be valued.
Experience with flow cytometry and FACS analysis is a plus.
Experience with biochemical/biophysical assays such as SPR, BLI, AUC, ITC, etc. is preferred.
Experience with identifying biomarkers using proteomics approaches and developing biomarker and PK assays for plasma proteins is a plus.
Background in one or more of the following: neurobiology, cardiac or skeletal muscle biology, metabolic or inflammatory diseases would be an asset.
Ability to work independently, design experiments, follow and adapt protocols, conduct, interpret, and optimize experimental studies is required.
Excellent verbal and written communication skills and clear scientific presentation skills during project and team meetings is required.
Must have excellent organizational and documentation skills. The candidate is expected to document all aspects of experiments performed, to conduct independent research, and to collect and archive all necessary information from the literature.
Must have excellent team working skills, adaptability/flexibility in a fast-paced research environment with “can-do” attitude.