Senior Scientist/Scientist/Associate Scientist, Protein Engineering – R&D

Company Description

Elevian is a well-funded early stage biotechnology company developing transformative therapeutics to address diseases of aging.  Elevian’s founders, Drs. Richard T. Lee, Amy Wagers and Lee Rubin, in the Harvard Department of Stem Cell Biology and Regenerative Biology (HSCRB) have identified specific circulation blood factors that promote regeneration in tissues that have been damaged by aging and degeneration. Mouse model studies involving supplementation of GDF11, a Transforming Growth Factor-Beta (TGF-b) ligand, have demonstrated improvements in age related cardiac hypertrophy, youthful skeletal muscle repair, neurogenesis and cognitive function, and Type II Diabetes.  This ground-breaking discovery was recognized by Science as a Global Top 10 Breakthrough of 2014.  Elevian is developing therapeutics that regulate GDF11 and other circulating factors that stimulate and replenish our body’s natural regenerative capacity to address multiple degenerative diseases. We are looking for well-qualified, enthusiastic, and motivated scientists who wish to join the Elevian team of serial entrepreneurs, biotech veterans, and world renown scientists in regenerative medicine and aging biology and contribute to our mission to develop therapeutics that improve “healthy aging”. Elevian’s research will be performed at The Pagliuca Harvard Life Lab and will enable close collaboration with the laboratories of its academic founders.

 

Position Description: Senior Scientist/ Scientist/Associate Scientist, Protein Engineering – R&D

We are seeking a dedicated, innovative, and motivated Senior Scientist, Scientist or Associate Scientist with extensive hands on experience in protein expression in mammalian and/or in other systems, protein purification, and protein characterization including the development and execution of cellular and biochemical activity and binding assays.   

Responsibilities of the successful candidate will include:

  • Design and assemble vectors for recombinant protein expression.

  • Produce protein in mammalian cells and in E. coli both internally and in collaboration with labs of academic founders and third party CROs.

  • Develop scalable expression and purification processes.

  • Support antibody discovery and development efforts.

  • Develop and implement assays to characterize protein activity and function in vitro.

  • Characterize basic biochemical and functional activity properties of recombinant proteins and antibodies in vitro and support qualification of candidates for in vitro and in vivo efficacy studies.

  • Protein expression in mammalian cells and stable cell line generation.

  • Design and engineer novel protein variants of Elevian’s therapeutic candidates.

  • Collaborate with labs of Elevian’s three academic founders at HSCRB and with third party CROs.

  • Contribute to the culture and team leadership at Elevian’s research labs at The Pagliuca Harvard Life Lab 

Qualifications

  • PhD(preferred) or MS with 2 - 4 years of relevant industry or academic experience.

  • Strong background in protein structural biology and biochemical and biophysical methods of protein characterization is a must.

  • A deep understanding of modern cloning approaches, rapid construct design, basic bioinformatics, and tagging strategies for the expression and purification of protein is required.

  • Demonstrated ability to implement a variety of construct designs, different expression strategies, and use of multiple expression systems is required.

  • Experience in purification of tagged and untagged proteins derived from various expression systems (mammalian, insect and bacterial).

  • Expertise in standard protein chromatography techniques on an AKTA FPLC (or similar) systems including affinity chromatography, IEX, SEC, and reverse-phase etc. is a must.

  • Experience with analyzing proteins (SE-HPLC, SDS-PAGE, Western Blots, and endotoxin assays) is required.

  • Experience with biochemical/biophysical assays such as SPR, BLI, AUC, ITC, etc. is highly desired.

  • Experience with mammalian cell culture and aseptic techniques is required.

  • Experience with transient and stable mammalian (CHO and HEK) protein expression is a plus.

  • Experience with cell-based activity-reporter assays is a plus.

  • Experience with standard molecular and cell biology techniques and assay technologies: PCR, QPCR, ELISA, FP, FRET, immunohistochemistry etc. is a plus.

  • Ability to work independently, design experiments, follow and adapt protocols, conduct, interpret, and optimize experimental studies is a must.

  • The candidate should have excellent verbal and written communication skills. Clear scientific presentation skills during project and team meetings is required.

  • Must have excellent organization skills. The candidate is expected to document all aspects of experiments performed and be able to conduct independent research to collect and archive all necessary information from the literature.

  • Excellent team working skills and adaptability are required.

Sharon Reaves